![]() Thirty hours after transfection, the cells were washed twice with PBS and lysed in RIPA buffer (PBS supplemented with 1% Nonidet P-40/0.5% sodium deoxycholate/1 mM PMSF/1 μg/ml aprotinin/1 mM sodium orthovanadate). For immunoprecipitation, 293 cells were transiently transfected by using the FuGENE 6 reagent (Roche Molecular Biochemicals) according to manufacturer protocol. Cell Culture and Immunoprecipitation.ĬOS-1 and 293 cells were maintained at 37☌ with 5% CO 2 in DMEM containing 10% FCS, 50 units/ml penicillin, and 50 μg/ml streptomycin. All constructs were confirmed by DNA sequencing. Mammalian expression vectors for N-terminally FLAG-tagged full-length (MTMR5-WT, amino acids 1–1,930), Δ-coiled coil (MTMR5-Δcoil, amino acids 1–1,640), and Δ-PH (MTMR5-ΔPH, amino acids 1–1,760) MTMR5 proteins were created by inserting cDNA fragments into the 5′ HindIII and 3′ EcoRI sites of pCDNA3.1-NF. ![]() The pGEX- MTMR5 vector was created by inserting the MTMR5 ORF into the 5′- EcoRI and 3′- HindIII sites. The complete MTMR5 ORF was amplified by PCR by using a pMSCVneo- sbf1 as a template ( 30). Vectors for the expression of bacterial recombinant GST-tagged MTMR5 was created by using the pGEX-6P-2 (Amersham Pharmacia). Site-directed mutagenesis was carried out by using a PCR-based procedure ( 29). A PCR product encoding protein fragments that correspond to the MTMR2-ΔPH domain (MTMR2-ΔPH, amino acids 183–643) and the MTMR2-Δcoiled-coil domain (MTMR2-Δcoil, amino acids 1–588) were inserted into the 5′ BglII and 3′ KpnI sites of pEGFP (CLONTECH). Bacterial expression vectors for recombinant His-tagged MTM1 and MTMR2 fusion proteins have also been described ( 7, 11). Vectors for mammalian expression of N-terminally FLAG-tagged MTM1 and MTMR2 and enhanced GFP (EGFP)-tagged MTM1 and MTMR2 fusion proteins have been described ( 7, 11). ![]()
0 Comments
![]() ![]() In “The Rings of Power,” we will presumably get to see some of these storied blades - the sword of Isildur (Maxim Baldry), for example, which is known as Narsil and is weighted with destiny. And sometimes a legendary sword can do more to fuel fear and awe than the individual wielding it. ’Tis said the sword makes the man - or the woman, or the elf. Rhaenyra wants only her birthright and perhaps there’s something heroic in fighting the patriarchy to get it, but so far she’s no Galadriel, even if the blonde wigs make the Targaryens resemble elves. As causes go, that’s not as noble as Galadriel’s quest to extinguish the ultimate evil, or even Dany’s early fight against oppression. Instead, “Dragon” implicitly asks viewers to identify Rhaenyra (Milly Alcock) with Daenerys (Emilia Clarke) and therefore support her claim for the throne. But that would have required wedging those characters into the story in places where they didn’t really fit. “Dragon” might have taken a similar route if the showrunners had been willing to revisit such long-living “Thrones” characters as Melisandre (Carice van Houten) or the Children of the Forest. Auden wrote about “The Fellowship of the Ring,” the first volume of Tolkien’s trilogy, in 1954. A Soviet Take: A 1991 production based on Tolkien’s novels, recently digitized by a Russian broadcaster, is a time capsule of a bygone era. ![]() Being Frodo: The actor Elijah Wood explains why he’ll never be upset at being associated with the “Lord of the Rings” movie series.He invented an alternate reality, complete with its own geography, languages and history. Artist and Scholar: Tolkien did more than write books.Tolkien, now adapted into a new series for Amazon Prime Video, has inspired generations of readers and viewers. Morfydd Clark, as an adventurous young Galadriel in “Rings” (played by Cate Blanchett in the movies) manages this quite nicely.Įxplore the World of the ‘Lord of the Rings’ The literary universe built by J.R.R. The trick, of course, is that new actors have to measure up to those playing previous incarnations, some of whom were widely beloved. But it’s still early, fan reaction to the end of “Thrones” was truly bitter, and the franchise still has a lot of ground to make up.Īs prequels go, “Rings of Power” has another advantage because some of its characters are immortal. ![]() If the monster ratings seen thus far for “Dragon” are any indication, “Thrones” fans seem prepared to forgive (if not forget) for now. Tolkien fans are already finding things to gripe about with the new series, but they’ve have had much more time to get over the “Hobbit” movies. Each series got off to a great start, but each tested viewers’ patience. It could be said that the early seasons of “Game of Thrones” were in some ways comparable to the first three (and much-loved) Jackson films, while the derided later seasons of “Thrones” resembled more the polarizing “Hobbit” movies. Tolkien has sold more books than Martin (they’ve both sold tens of millions), but Tolkien’s have been around much longer.Ī better comparison might be the previous adaptations of their work: HBO’s “Game of Thrones,” to which “Dragon” is a prequel, versus Peter Jackson’s film versions of “The Lord of the Rings” and “The Hobbit.” Martin takes inspiration from much of what Tolkien did, especially in the areas of magic and world-building but he has also expanded on Tolkien’s achievements. Martin, who is often referred to as “the American Tolkien.” The two authors are not in competition. (Some spoilers lie ahead.) Pop culture cred But we’ve seen enough to get the discussion started. Which one has the coolest swords? The best dragons? The most formidable heroine? Granted, initial observations are based on only the first few episodes (three so far for “Dragon” two for “Rings,” which premiered on Thursday). Instead of comparing industry stats, though - ratings, budgets, and so on - let’s look at where the two shows overlap. “We don’t have to be bracketed together.” ![]() “It’s not a death match or anything,” he told The Hollywood Reporter. Martin - whose work was heavily influenced by the original “Rings” author, J.R.R. And indeed the internet has already been more than happy to oblige.īut should we compare them? Possibly not. Comparisons between HBO’s “House of the Dragon” and Amazon’s “The Lord of the Rings: The Rings of Power” - both new epic fantasies, both prequel series, both with huge budgets and ready-made fan bases - were probably inevitable. ![]() ![]() ![]() ![]() GPG, timestamped, and a message attached.Following the philisophy of git, branching is an essential and much used part of the development procedure. Use annotated tags since they contain the author and can be cryptographically signed using There are two basic types of tags: annotated and lightweight. Point in time, like for instance versions (have a look at semantic versioning, We use tags to record particular states or milestones of a project at a given The commit history looks as if the rebased commits were all doneĪ tag is a pointer to a commit but in contrast to a branch it does not ever move. This also demonstrates that git rebase is a command that alters History is now linear and does not contain merge commits.Īll the commit hashes that were on the branch that got rebased, haveĬhanged. Which we will learn to resolve in the next section. If you give them a go, keep in mind that you might run into conflicts, ![]() The following exercises are advanced, absolutely no problem to postpone them to aįew months later. May have a hard time finding them as there is no branch pointing to them. ![]() Even then your commits will not be lost but you Git will not let you delete a branch which has not been reintegrated unless you Only the pointers (“sticky notes”) disappeared, not the commits. * 4f00317 (HEAD -> master) Merge branch 'less-salt' |\ | * bf59be6 reduce amount of salt * | c43b24c Merge branch 'experiment' |\ \ | * | 6feb49d maybe little bit less cilantro | * | 7cf6d8c let us try with some cilantro | |/ * | 40fbb90 draft a readme |/ * dd4472c we should not forget to enjoy * 2bb9bb4 add half an onion * 2d79e7e adding ingredients and instructionsĬomparing figures “Commit graph after merge”Īnd “Commit graph after merged branches were deleted”, “fast sequence extraction” or “Python interface” or “fixing bug in There are different branching strategies, but it is useful to think that a branch Other than this convention there is nothing special about master or main, it is just a branch.Ĭommits form a directed acyclic graph (we have left out the arrows to avoid confusion about the time arrow).Ī group of commits that create a single narrative are called a branch. Main line development is often called master or main. We see branching points and merging points. The strength of version control is that it permits the researcher to isolateĭifferent tracks of work, which can later be merged to create a composite We need to be able to separate different lines of work really well. We typically need at least one version of the code to “work” (to compile, to give expected results, …).Īt the same time we work on new features, often several features concurrently. Software development is often not linear: When we talk about branches, we often mean all parent commits, not only the commit pointed to. “HEAD” is the current position (remember the recording head of tape recorders?). Here the branch master points to a commit. Up until now our repository had only one branch with one commit comingĬommits are depicted here as little boxes with abbreviated hashes. In the previous section we tracked a guacamole recipe with Git. ![]() ![]() But first, let's clarify one important thing. Using the proper email client for your needs can decrease time spent on email while increasing the results you get from using email.Īn email client specifically designed for the Mac is a great tool to have in your toolbox. No matter how many new messaging apps enter your workflow, email will likely always be a part of your professional life. When you find an email client that can deliver on all of the above, you have a great opportunity to reduce the amount of time you spend in your inbox.Īs Abraham Lincoln said, "Give me six hours to chop down a tree, and I will spend the first four sharpening the ax." Ease of useĭoes your email client provide features that make email management better for you while also remaining accessible? An effective email client needs to strike a balance between customization and ease of use. SecurityĪre your private emails being stored without satisfactory security precautions or used for targeted advertising? They shouldn't be! Ecosystemĭoes your email client also work on the web or on the go? Can it integrate with other systems like calendars and web conferencing platforms? Email clients should work with your other systems, not against them. ![]() The average person spends three hours per day in their inbox does your email client provide a pleasant environment in which to operate? Your office environment (including your screen) plays a significant role in measurable productivity. After all, if you're a Mac user you're likely going to want your email client to operate in a similar manner as your operating system, right? How we evaluate Mac email clients Qualityĭoes your email client help you get your email tasks done more effectively and efficiently? Let's examine the different attributes that contribute to being productive in your inbox. That said, it makes sense to use the same criteria that most people use to compare computers (Mac vs. Mac users generally are willing to accept that Apple has put enough effort and expertise into the design to not require them to customize every little detail. PC users are accustomed to having the ability to edit every detail of their machine to their workflows and individual preferences. This is opposed to a feature like customization, which is a huge draw for PC enthusiasts. Most Apple users purchase their equipment for a few distinct reasons: So what makes an email client especially well suited for the Mac? The same qualities that distinguish Macs from other computer systems. Technically, Microsoft Outlook has a Mac email client, but if you're reading this blog post from an Apple device and don't have an obligation from your employer to use it, that's probably not what you're looking for… ![]() Finding the best email client for Mac is more than just platform compatibility. ![]() ![]() With 12 speakers, an 11-channel amplifier, and 630 watts of decibel-pumping power, the setup should give your ears a jolt during any jaunt. Perfectly tuned for the road, the 2021 Santa Fe sound system is a Harman-Kardon powerhouse. The available tablet-sized screen is maybe the best way to experience one of the industry’s best infotainment systems. Replacing the 2020 Santa Fe’s available 8.0-inch screen is an all-new, high-definition 10.25 widescreen display. ![]() ![]() Who couldn’t use an extra inch? Available 10.25-in Widescreen Display with Navigation Mathematically, the new 2021 Santa Fe touchscreen (8 in/20 cm) is a 14% upgrade over the 2020 model’s 7.0-inch (18 cm) screen. Standard 8.0-in Touchscreen (Replaces the 7.0-in Display) Many new technological additions and improvements (see below).Integrated Memory System with Radio and HVAC Control now available.Available Eco-suede headliner and door panels are new.New quilted Nappa leather seats and upholstery. ![]() Boy, we can’t wait for our favorite SUV to arrive-can’t you tell? Interior & Tech Updates Refreshed but still rocking that fierce 2020 Hyundai Santa Fe spirit, the 2021 model comes slathered with new driver safety and convenience features. (That means, “read more below.”) 2020 vs. You can pick up the new Santa Fe at our Olathe Hyundai dealership when it’s released later this fall, but if you simply cannot wait to get to know your next SUV, carry on my Santa Fe-ward son. Of course, we’re talking about the 2021 Hyundai Santa Fe, whose official specs and features just hit the interweb. ![]() ![]() I showed it to my team, but the idea was tabled for awhile because we thought it through and didn’t want people buried in their phones while singing. ![]() That web server would then relay those changes to all of the connected clients, much like a chat server would send a message to everyone. I created a local Node.js project and in just a few hours, I had something ready to alpha test! My approach was to have a web browser open on the local network that could poll and listen to changes from ProPresenter, and then relay that new data to a web server. Basically, by using websockets, we can interact with ProPresenter which will return JSON-formatted data reflecting information about songs in the library, playlist, the index of the current song, the current slide and next slide information, etc. Some people have done a great job at figuring out how ProPresenter sends data over the network between their apps which allows us to extend the software to meet unique needs. I say undocumented because it is not officially offered as a way to access ProPresenter data and control it. It needed to be real time or as close to it as possibleĪwhile back, I started tinkering around with the undocumented ProPresenter API.It needed to work in the standard phone browser so there was no barrier of installing a particular app.So, I had an idea: What if we could somehow send the lyrics out of ProPresenter to everyone’s phones, in real time, and let them use their own screens to follow along? Pretty small (60″) for such a large crowd. Of course, it’d be great if we could just rent an LED video wall, but the cost to do that has been too expensive for us to do in the past. Big white letters on a black background, nothing fancy. ![]() Because it doesn’t get dark enough to use projectors for lyrics until the service is almost over, in the past we have relied on using small flat screen TVs to try to show some words for people to follow along. Every year, my church has a “night of worship”, a worship service in the heart of the city at an outdoor stage, where we sing songs for a couple of hours. ![]() ![]() ![]() We apologize in advance for the inconvenience. ![]() Trova finestra magica giocattolo unici, accattivanti e a prezzi competitivi. It wasnt the greatest toy in the world, but it was fun to mess with once in a while. Con finestra magica giocattolo di, offri ai bambini un intrattenimento sano. Support your childs learning during important developmental windows with play essentials that are tailored to their exact stage. Janu843 views I saw a Magic Window when I was sitting in a small waiting room around 1973 or 1974 and thought it was kind of fun, so I eventually got one.During that time period, TSDR will not be able to provide assignment information. Wham-O Magic Window Toy Sign in to follow this Followers 1 Entry posted by Random Terrain WHAM-O MAGIC WINDOW Blue and White Sand Art Toy 70s Vintage NO STAND Pre-Owned C 113.86 Top Rated Seller or Best Offer +C 18.34 shipping estimate from United States Results matching fewer words Vintage 1 Bottle WHAM-O Magic Sand 1981 New Brand New C 50. For assistance with TSDR, email and include your serial number, the document you are looking for, and a screenshot of any error messages you have received.Īssignments on the Web (AOTW) will be unavailable between 05:00am and 01:00pm EST. We apologize in advance for the inconvenience. Get the best deals for magic window toy at. During that time period, TSDR will not be able to provide assignment information. Disney on Ice is a series of touring ice shows produced by Feld Entertainment under agreement with The Walt Disney Company. See current trademark processing wait times for more information.Īssignments on the Web (AOTW) will be unavailable between 05:00am and 01:00pm EST. Cloutier, and licensed to Whamo sold over 20 million units in the 1970s. which should make good window and counter displays. Processing Wait Times: Please note that due to an extraordinary surge in applications, processing times are longer than usual. The 1922 Toy Fair Tly a reke Hotels imperial and Breslin, New Tediaus Pastimes - Over Sunday in. Trademark Law: Rules of Practice & Federal Statutesįor assistance with TSDR, email and include your serial number, the document you are looking for, and a screenshot of any error messages you have received. ![]() ![]() At MCC, the video and audio are recombined and sent to Maryland, where it is then streamed to YouTube. Get the latest updates on NASA missions, watch NASA TV live, and learn about our quest to reveal the unknown and benefit all humankind. The video is sent from ISS to MCC via Ku-Band and the audio is sent via S-Band. brings you the latest images, videos and news from America's space agency. The return link used to peak at 50 Mbps.Īll that said, the ISS doesn't stream live to Youtube. ![]() I should also mention that the 25/300 Mbps link is a recent improvement. That's why the ISS Internet has been described as slow. Once the signal gets picked up by the ISS comm system, it is reformatted and sent to the LAN and out to the laptop. And again, the “Internet” data only gets a portion of that bandwidth. However, while the return link was up to 300 Mbps, the forward link is up to 25 Mbps. The site in New Mexico beams that data up 30,000 plus km to the TDRS satellite, which then beams it to ISS. Software on that computer captures the resulting images and passes them through multiple servers, back out to New Mexico. The app utilizes the native Android Google Map (ISS tracker), which allows you to follow the Space Stations orbit around our planet. That page data fills the screen of the ground computer. On that computer, the cursor scrolls and clicks on the link, making a request through regular commercial Internet pathways to pull the page data. What is actually happening is that your request to move the cursor and click on a link is being transmitted as commands from the laptop to the onboard server to the comm system across more than 30,000 km of space to a satellite that then relays that signal another more than 30,000 km to a site in New Mexico that then relays the signal through ground lines to Mission Control, through more servers and eventually to a computer that is actually connected to the Internet. Live Feeds from the International Space Station AND near-live timelapse videos of Earth from geosynchronous orbit. You scroll your cursor across the screen and click on a link. Imagine you are an astronaut onboard the ISS and you have a webpage up on your laptop. Let's talk through an example to show how it works. ![]() For cyber security purposes, the Internet connection is simply a mirroring of computers on the ground. There is not a direct Internet connection to the ISS. ![]() ![]() I.Ps that have been used to shape video game history are used to produce non-working versions that are still segregated into micropayment and pay-wall loaded profit gardens for anyone dumb enough to consider the tempt of wanting to live over a specific section of their past. Right here, you’ll be able to head back to Origin.Ī familiar face in the crowd, Ea reveals a corporation whose history is one of eating the brains from former (healthy) firms and implanting them into the bodies of spacecraft Troopers everywhere they are found. For a just $5.99, you can get your hands on one of the most popular computer games of all time. The city-building game is currently available for free on EA’s Origin digital store, but it is unclear how long the offer will last, so you should probably get it now. Simcity 2000 Download: Still resentful of last year’s disastrous debut of the new SimCity? The good news is that you can make up for it right away by downloading the original SimCity 2000 for free. Head to the transfer link in the footer of this post to copy it to your laptop. and HAL Laboratory are two of the game’s developers, while Electronic Arts and Maxis are its publishers. This may be a (City-building Simulation Video) game for (Macintosh OS, DOS, Amiga Microsoft Windows Various SNES Sega Saturn PlayStation N64 GBA PSN OS/2 OS/2) Destination software system, Inc. In the current version, it has a near-isometric dimetric read (similar to the earlier Maxis-published A-Train) instead of overhead, land may have completely varied heights, and underground layers were created for water lines and subways. In SimCity 2000, the concept was further developed. ![]() In light of the original success of SimCity and the general lack of success with other “Sim” titles, a sequel was inevitable. Simcity 2000 Download: A city-building simulation computer game, SimCity 2000 is the second chapter in the series. ![]() ![]() Click on the Speed drop-down menu and change Normal to Slow.Ħ) Your video will then be slowed down to half-speed, which doubles the video length. Once you do, click on the video to select it, and then click on the Speed button:ĥ) This will show the speed-editing interface. To do that, click on the Projects button.Ĥ) In the prompt, give your project a name, and then click on the blue OK button.ĥ) Now that your project is created, you can begin editing the video by dragging it into your timeline. If you have iMovie on your Mac, just follow the steps below and you’ll have a slow motion videos in no time:ġ) Launch iMovie on your Mac, and then click on the Import Media button.Ģ) In the file browser, select the video file you want to convert to slow motion, then click on the blue Import Selected button.ģ) Now the file is added to iMovie. Using iMovie to convert videos to slow motion If you haven’t bought a Mac recently, you’ll have to shell out $14.99 for the app. When I got home, I wanted to share the lightning I captured on video on Facebook with my friends and family, but I wanted to slow the video down so the lightning was easier to see.įor this, I used iMovie, a free download from the Mac App Store for anyone who has bought a Mac recently. One night it just happened to be raining cats and dogs outside, and Florida lightning can get pretty intense. Fortunately, iMovie for Mac can convert your videos into slow motion, and in this tutorial, we’ll show you how.įor example, I have a dash camera in my vehicle that I like to use and grab footage from, but as you can imagine, it doesn’t record in slow motion. On the other hand, if you don’t use your iPhone to capture video, getting the video files from your other devices to transform into slow motion is a little trickier. ![]() You can record videos in 120 or 240 frames per second and then watch it play back in a much slower manner than it typically would. Select the speed of the slow-motion with the slider at the top of the window.ĭrag the blue mark (or parks, if you choose a faster speed) to the points you want the slo-mo to start and finish.Ĭlick Save the copy to create a new video file with all the implemented changes.Slow motion videos are one of the fun aspects of using your iPhone for videogprahy. Right-click the video you want to edit, choose Open with → Photos. The app cannot make the video faster its only speed-changing feature is slo-mo. If yoг want to create a slow-motion effect on your video, use the Photos app. Right-click the app’s playback area, choose Enhancements → Play speed settings.ĭrag the speed slider to the desired position. It’s the default player for Windows 10, so you don’t have to install anything additionally. If you want to change the playback speed of your video, use Windows Media Player. How do I change the speed of a video in Windows 10? If you change your mind, you can restore the Slow Motion effect in the video by putting the drag bars back. The speed of your video is now back to normal. This will make the Slow Motion range disappear. To make the speed normal again, drag the bar on the left to the right all the way to the end of the clip. You can identify the Slow Motion section by large drag bars at either end. On the Timeline, you will see the range of the video shot in Slow Motion. ![]() ![]() ![]() Tap Slo-Mo to reveal the list of videos shot with this effect and select a video to speed up. Launch the Photos app and select Albums at the bottom of the screen. Here’s a short guide on how to fast-forward a video using the app pre-installed on every iPhone (and iPad). For a video recorded with the native Slo-Mo effect in your Camera, just use the Photos app. If you simply want to restore a slow-motion video’s speed to normal using your iPhone, you won’t need an additional video accelerator. How to bring a slow-motion video back to normal? ![]() |